<?xml version="1.0" encoding="UTF-8"?>
<!--This biomodel was generated in VCML Version 0.4-->
<vcml xmlns="http://sourceforge.net/projects/vcell/vcml" Version="0.4">
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      <Model Name="unnamed">
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</Annotation>
         <Compound Name="E" />
         <Compound Name="S" />
         <Compound Name="P" />
         <Compound Name="ES" />
         <Feature Name="N" KeyValue="33248978" />
         <LocalizedCompound Name="E_N" CompoundRef="E" Structure="N" OverrideName="false" KeyValue="33248980" />
         <LocalizedCompound Name="S_N" CompoundRef="S" Structure="N" OverrideName="false" KeyValue="33248981" />
         <LocalizedCompound Name="P_N" CompoundRef="P" Structure="N" OverrideName="false" KeyValue="33248982" />
         <LocalizedCompound Name="ES_N" CompoundRef="ES" Structure="N" OverrideName="false" KeyValue="33248983" />
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            <Reactant LocalizedCompoundRef="E_N" Stoichiometry="1" KeyValue="33248985" />
            <Reactant LocalizedCompoundRef="S_N" Stoichiometry="1" KeyValue="33248986" />
            <Product LocalizedCompoundRef="ES_N" Stoichiometry="1" KeyValue="33248987" />
            <Kinetics KineticsType="GeneralKinetics">
               <Parameter Name="J" Role="reaction rate" Unit="uM.s-1">((k1 * E_N * S_N) - (k2 * ES_N) - (k3 * ES_N))</Parameter>
               <Parameter Name="k1" Role="user defined" Unit="uM-1.s-1">1.0</Parameter>
               <Parameter Name="k2" Role="user defined" Unit="s-1">0.5</Parameter>
               <Parameter Name="k3" Role="user defined" Unit="s-1">1.0</Parameter>
            </Kinetics>
            <Annotation />
         </SimpleReaction>
         <SimpleReaction Structure="N" Name="reaction1" FluxCarrierValence="0" FluxOption="MolecularOnly" KeyValue="33248988">
            <Reactant LocalizedCompoundRef="ES_N" Stoichiometry="1" KeyValue="33248989" />
            <Product LocalizedCompoundRef="P_N" Stoichiometry="1" KeyValue="33248990" />
            <Product LocalizedCompoundRef="E_N" Stoichiometry="1" KeyValue="33248991" />
            <Kinetics KineticsType="GeneralKinetics">
               <Parameter Name="J" Role="reaction rate" Unit="uM.s-1">(k3 * ES_N)</Parameter>
               <Parameter Name="k3" Role="user defined" Unit="s-1">1.0</Parameter>
            </Kinetics>
            <Annotation />
         </SimpleReaction>
         <Diagram Name="Unnamed compartment" Structure="N">
            <LocalizedCompoundShape LocalizedCompoundRef="E_N" LocationX="67" LocationY="172" />
            <LocalizedCompoundShape LocalizedCompoundRef="S_N" LocationX="54" LocationY="68" />
            <LocalizedCompoundShape LocalizedCompoundRef="P_N" LocationX="430" LocationY="146" />
            <LocalizedCompoundShape LocalizedCompoundRef="ES_N" LocationX="236" LocationY="89" />
            <SimpleReactionShape SimpleReactionRef="reaction0" LocationX="134" LocationY="91" />
            <SimpleReactionShape SimpleReactionRef="reaction1" LocationX="312" LocationY="143" />
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      </Model>
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</Annotation>
            <Extent X="10.0" Y="10.0" Z="10.0" />
            <Origin X="0.0" Y="0.0" Z="0.0" />
            <SubVolume Name="Compartment" Handle="0" Type="Compartmental" KeyValue="33248967" />
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         </Geometry>
         <GeometryContext>
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               <BoundariesTypes Xm="Flux" Xp="Flux" Ym="Flux" Yp="Flux" Zm="Flux" Zp="Flux" />
            </FeatureMapping>
         </GeometryContext>
         <ReactionContext>
            <LocalizedCompoundSpec LocalizedCompoundRef="E_N" ForceConstant="false" EnableDiffusion="true">
               <InitialConcentration>2.0</InitialConcentration>
               <Diffusion>0.0</Diffusion>
            </LocalizedCompoundSpec>
            <LocalizedCompoundSpec LocalizedCompoundRef="S_N" ForceConstant="false" EnableDiffusion="true">
               <InitialConcentration>10.0</InitialConcentration>
               <Diffusion>0.0</Diffusion>
            </LocalizedCompoundSpec>
            <LocalizedCompoundSpec LocalizedCompoundRef="P_N" ForceConstant="false" EnableDiffusion="true">
               <InitialConcentration>0.0</InitialConcentration>
               <Diffusion>0.0</Diffusion>
            </LocalizedCompoundSpec>
            <LocalizedCompoundSpec LocalizedCompoundRef="ES_N" ForceConstant="false" EnableDiffusion="true">
               <InitialConcentration>0.0</InitialConcentration>
               <Diffusion>0.0</Diffusion>
            </LocalizedCompoundSpec>
            <ReactionSpec ReactionStepRef="reaction0" ReactionMapping="included" />
            <ReactionSpec ReactionStepRef="reaction1" ReactionMapping="included" />
         </ReactionContext>
         <MathDescription Name="ode_generated">
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</Annotation>
            <Constant Name="_F_">96480.0</Constant>
            <Constant Name="_F_nmol_">9.648E-5</Constant>
            <Constant Name="_K_GHK_">1.0E-9</Constant>
            <Constant Name="_N_pmol_">6.02E11</Constant>
            <Constant Name="_R_">8314.0</Constant>
            <Constant Name="_T_">300.0</Constant>
            <Constant Name="E_N_init">2.0</Constant>
            <Constant Name="ES_N_init">0.0</Constant>
            <Constant Name="k1">1.0</Constant>
            <Constant Name="k2">0.5</Constant>
            <Constant Name="k3_reaction0">1.0</Constant>
            <Constant Name="k3_reaction1">1.0</Constant>
            <Constant Name="K_millivolts_per_volt">1000.0</Constant>
            <Constant Name="KMOLE">0.0016611295681063123</Constant>
            <Constant Name="P_N_init">0.0</Constant>
            <Constant Name="S_N_init">10.0</Constant>
            <Constant Name="Size_N">1.0</Constant>
            <VolumeVariable Name="ES_N" />
            <VolumeVariable Name="P_N" />
            <Function Name="E_N">(K_E_N_total - ES_N)</Function>
            <Function Name="J_reaction0">((k1 * E_N * S_N) - (k2 * ES_N) - (k3_reaction0 * ES_N))</Function>
            <Function Name="J_reaction1">(k3_reaction1 * ES_N)</Function>
            <Function Name="K_E_N_total">(E_N_init + ES_N_init)</Function>
            <Function Name="K_S_N_total">(ES_N_init + S_N_init + P_N_init)</Function>
            <Function Name="S_N">( - ES_N + K_S_N_total - P_N)</Function>
            <CompartmentSubDomain Name="Compartment">
               <BoundaryType Boundary="Xm" Type="Value" />
               <BoundaryType Boundary="Xp" Type="Value" />
               <BoundaryType Boundary="Ym" Type="Value" />
               <BoundaryType Boundary="Yp" Type="Value" />
               <BoundaryType Boundary="Zm" Type="Value" />
               <BoundaryType Boundary="Zp" Type="Value" />
               <OdeEquation Name="P_N" SolutionType="Unknown">
                  <Rate>J_reaction1</Rate>
                  <Initial>P_N_init</Initial>
               </OdeEquation>
               <OdeEquation Name="ES_N" SolutionType="Unknown">
                  <Rate>(J_reaction0 - J_reaction1)</Rate>
                  <Initial>ES_N_init</Initial>
               </OdeEquation>
            </CompartmentSubDomain>
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               <GroupAccess Type="1" />
               <Annotation>cloned from 'ode_generated' owned by user satarupa
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            </Version>
         </MathDescription>
         <Simulation Name="Simulation0">
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</Annotation>
            <SolverTaskDescription TaskType="Unsteady" UseSymbolicJacobian="false" Solver="IDA (Variable Order, Variable Time Step, ODE/DAE)">
               <TimeBound StartTime="0.0" EndTime="5.0" />
               <TimeStep DefaultTime="0.1" MinTime="1.0E-8" MaxTime="1.0" />
               <ErrorTolerance Absolut="1.0E-9" Relative="1.0E-9" />
               <OutputOptions KeepEvery="1" KeepAtMost="1000" />
            </SolverTaskDescription>
            <MathOverrides>
               <Constant Name="S_N_init">100.0</Constant>
               <Constant Name="k2">1.0</Constant>
            </MathOverrides>
            <Version Name="Simulation0" KeyValue="33249003" BranchId="33249004" Archived="0" Date="17-May-2009 20:20:00" FromVersionable="false">
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               <Annotation>cloned from 'Simulation0' owned by user satarupa
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         </Simulation>
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            <SolverTaskDescription TaskType="Unsteady" UseSymbolicJacobian="false" Solver="IDA (Variable Order, Variable Time Step, ODE/DAE)">
               <TimeBound StartTime="0.0" EndTime="5.0" />
               <TimeStep DefaultTime="0.1" MinTime="1.0E-8" MaxTime="1.0" />
               <ErrorTolerance Absolut="1.0E-9" Relative="1.0E-9" />
               <OutputOptions KeepEvery="1" KeepAtMost="1000" />
            </SolverTaskDescription>
            <MathOverrides>
               <Constant Name="S_N_init">100.0</Constant>
               <Constant Name="E_N_init">1.0</Constant>
               <Constant Name="k3_reaction1">5.0</Constant>
               <Constant Name="k2">0.01</Constant>
               <Constant Name="k3_reaction0">5.0</Constant>
            </MathOverrides>
            <Version Name="Simulation1" KeyValue="33249006" BranchId="33249007" Archived="0" Date="17-May-2009 20:20:00" FromVersionable="false">
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               <GroupAccess Type="1" />
               <Annotation>cloned from 'Simulation1' owned by user satarupa
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            </Version>
         </Simulation>
         <Simulation Name="Simulation2">
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</Annotation>
            <SolverTaskDescription TaskType="Unsteady" UseSymbolicJacobian="false" Solver="IDA (Variable Order, Variable Time Step, ODE/DAE)">
               <TimeBound StartTime="0.0" EndTime="10.0" />
               <TimeStep DefaultTime="0.1" MinTime="1.0E-8" MaxTime="1.0" />
               <ErrorTolerance Absolut="1.0E-9" Relative="1.0E-9" />
               <OutputOptions KeepEvery="1" KeepAtMost="1000" />
            </SolverTaskDescription>
            <MathOverrides>
               <Constant Name="S_N_init" ConstantArraySpec="1000">100.0, 200.0, 300.0, 400.0, 500.0, 600.0, 700.0, 800.9, 100.0</Constant>
               <Constant Name="E_N_init">1.0</Constant>
               <Constant Name="k2">1.0</Constant>
            </MathOverrides>
            <Version Name="Simulation2" KeyValue="33249009" BranchId="33249010" Archived="0" Date="17-May-2009 20:20:00" FromVersionable="false">
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               <GroupAccess Type="1" />
               <Annotation>cloned from 'Simulation2' owned by user satarupa
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         </Simulation>
         <Simulation Name="Simulation3">
            <Annotation>cloned from 'Simulation3' owned by user satarupa
s=1, E=1, k1=k2=k3=1</Annotation>
            <SolverTaskDescription TaskType="Unsteady" UseSymbolicJacobian="false" Solver="IDA (Variable Order, Variable Time Step, ODE/DAE)">
               <TimeBound StartTime="0.0" EndTime="10.0" />
               <TimeStep DefaultTime="0.1" MinTime="1.0E-8" MaxTime="1.0" />
               <ErrorTolerance Absolut="1.0E-9" Relative="1.0E-9" />
               <OutputOptions KeepEvery="1" KeepAtMost="1000" />
            </SolverTaskDescription>
            <MathOverrides>
               <Constant Name="S_N_init">1.0</Constant>
               <Constant Name="E_N_init">1.0</Constant>
               <Constant Name="k2">1.0</Constant>
            </MathOverrides>
            <Version Name="Simulation3" KeyValue="33249012" BranchId="33249013" Archived="0" Date="17-May-2009 20:20:00" FromVersionable="false">
               <Owner Name="Education" Identifier="30625624" />
               <GroupAccess Type="1" />
               <Annotation>cloned from 'Simulation3' owned by user satarupa
s=1, E=1, k1=k2=k3=1</Annotation>
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         </Simulation>
         <Version Name="ode" KeyValue="33248993" BranchId="33248994" Archived="0" Date="17-May-2009 20:20:00" FromVersionable="false">
            <Owner Name="Education" Identifier="30625624" />
            <GroupAccess Type="1" />
            <Annotation>cloned from 'ode' owned by user satarupa
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         </Version>
      </SimulationSpec>
      <Version Name="michaelismenten_new" KeyValue="33249015" BranchId="33249016" Archived="0" Date="17-May-2009 20:20:00" FromVersionable="false">
         <Owner Name="Education" Identifier="30625624" />
         <GroupAccess Type="1" />
         <Annotation>cloned from 'michaelismenten_new' owned by user satarupa
</Annotation>
      </Version>
   </BioModel>
</vcml>

